SF3B3抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
SF3B3抗体

SF3B3抗体

商家询价

产品名称： SF3B3抗体

英文名称： SF3B3Antibody

产品编号： XY14577-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 SF3B3抗体Applications

In-house applications ELISA, WB, IHC, IF, IP     Application not tested? Please see our Guarantee.
Published applications WB Publications 3 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 14577-1-AP;HeLa cellHeLa cells were subjected to SDS PAGE followed by western blot with 14577-1-AP(SF3B3 antibody) at dilution of 1:10000
  • 14577-1-AP;GliomasImmunohistochemical of paraffin-embedded human gliomas using 14577-1-AP(SF3B3 antibody) at dilution of 1:100 (under 40x lens)
  • 14577-1-AP;HelaImmunofluorescent analysis of Hela cells, using SF3B3 antibody 14577-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
  • 14577-1-AP;rat brain tissueIP Result of anti-SF3B3 (IP:14577-1-AP, 4ug; Detection:14577-1-AP 1:5000) with rat brain tissue lysate 4800ug.
Positive WB detected in HeLa cells, human brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected in Rat brain tissue
Positive IHC detected in Human gliomas tissue
Positive IF detected in Hela cells
Recommended dilution WB : 1:1000-1:10000
IP : 1:2000-1:20000
IHC : 1:20-1:200
IF : 1:20-1:200
 SF3B3抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information SF3B3抗体
Immunogen SF3B3 fusion protein ag5980 Full name Splicing factor 3b, subunit 3, 130kDa
Calculated molecular
weight		 136 kDa Observed molecular 
weight		 130kd
GenBank accession 
number		 BC003146 Gene ID (NCBI) 23450
Gene symbol SF3B3 Synonyms KIAA0017, RSE1, SAP 130, SAP130, SF3b130, SF3B3, Splicing factor 3B subunit 3, STAF130
Background

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA. SF3B3抗体

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