TRIM25抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
TRIM25抗体

TRIM25抗体

商家询价

产品名称： TRIM25抗体

英文名称： TRIM25Antibody

产品编号： XY12573-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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 TRIM25抗体Applications

In-house applications ELISA, WB, IF, IP     Application not tested? Please see our Guarantee.
Published applications WB Publications 1 citations –view here
Species specificity Human, Mouse, Rat; other species not tested.
Published Species Mouse
Images
Prev1NextView more validation data
  • 12573-1-AP;MCF7 cellsMCF7 cells were subjected to SDS PAGE followed by western blot with 12573-1-AP(TRIM25 antibody) at dilution of 1:500
  • 12573-1-AP;HelaImmunofluorescent analysis of Hela cells, using TRIM25 antibody 12573-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
  • 12573-1-AP;K-562 cellsIP Result of anti-TRIM25 (IP:12573-1-AP, 4ug; Detection:12573-1-AP 1:600) with K-562 cells lysate 3200ug.
Positive WB detected in MCF7 cells, Jurkat cells, rat spleen tissue
Positive IP detected in K-562 cells
Positive IF detected in Hela cells
Recommended dilution WB : 1:200-1:2000
IP : 1:200-1:2000
IF : 1:20-1:200
 TRIM25抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information TRIM25抗体
Immunogen TRIM25 fusion protein ag3241 Full name Tripartite motif-containing 25
Calculated molecular
weight		 630aa,71 kDa Observed molecular 
weight		 71 kDa
GenBank accession 
number		 BC016924 Gene ID (NCBI) 7706
Gene symbol TRIM25 Synonyms EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147
Background

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25抗体

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