Product: Anti-CD138, clone MI15
Cat. No.: MC-418 (1 mL)
Synonym:
Sydencan-1
Background:
CD138 is a transmembrane heparin sulphate
proteoglycan which is made up of one core
protein and five glycosaminoglycans. CD-138 is
expected to play a role in cell adhesion. It is
expressed on the surface of pre-B cells and
plasma cells but is absent from mature B cells. It
is a selective marker for B cell lymphoblastic
leukemia and lymphoplasmocytoid leukemia. It
is lost from the apoptotic myeloma cells; hence
is a useful marker for viable myeloma cells.
Species Reactivity:
Human. Other species not tested.
Ig Isotype:
Mouse IgG1/k
Immunogen:
U-266 cells
Mol. Wt. of Antigen:
30.5 kDa (core protein), 85-92 kDa (depending
on glycosaminoglycans)
Epitope:
Ectodomain
Positive Control:
U-266 cells, tonsil
Cellular Location:
Cell membrane
Format:
1 mL of tissue culture supernatant with 0.09%
sodium azide.
Storage:
Store at 4oC.
Applications and Suggested Dilutions:

Immunoprecipitation

Western blotting

Immunohistochemistry: (Formalin/paraffin)
Use at a 1:10-1:20 dilution for 30 minutes at
room temperature. Staining of formalin-fixed
tissue REQUIRES boiling tissue sections in
10 mM citrate buffer, pH 6.0, for 10-20
minutes followed by cooling for 20 minutes at
room temperature.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.