Description

Mouse Embryonic Fibroblasts (MEF) are used to support the growth of mouse and human pluripotent stem cells [1]. MEF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. MEF are isolated from mouse embryos and used at early passages [2]. To serve as feeder cells, MEF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells 

 

MEF-mt from ScienCell Research Laboratories are isolated from embryonic day 13 CF1 mouse embryos. These cells have been treated with mitomycin C to prevent further cell division. They are cryopreserved at passage 3 and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. MEF-mt are characterized by immunofluorescence with antibody specific to fibronectin. MEF-mt are negative for mycoplasma, bacteria, yeast, and fungi. MEF are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MEF-mt are not recommended for expanding as mitomycin C treatment prevents further cell proliferation.

 

Recommended Medium

It is recommended to use DMEM (Cat. No. 09221) containing 10% fetal bovine serum (FBS, Cat. No. 0010, 0025, 0500) for the culturing of MEF-mt cells in vitro.

 

Product Use

MEF-mt are used as feeder layer in mouse and human pluripotent stem cell culture. They are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

 

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

 

Shipping

Dry ice.

 

Reference

[1] Bradley A. (1987) “Production and analysis of chimaeras.” In Robertson EJ, Teratocarcinomas and Embryonic Stem Cells: A Practical Approach (pp 113-51). Oxford: IRL Press.

[2] Nagy A, Gertsenstein M, Vintersten K, Behringer R. (2006) “Preparing Mouse Embryo Fibroblasts.” Cold Spring Harbor Protocols. pdb.prot 4398.